NSE (ENO2) Mouse Monoclonal Antibody [Clone ID: E27]

CAT#: AM00578SU-N

NSE (ENO2) mouse monoclonal antibody, clone E27, Supernatant

Product Images

Formalin-Fixed, Paraffin-Embedded Human pancreas stained with NSE antibody Cat.-No. AM00578SU-N using peroxidase conjugate and DAB chromogen. Note cytoplasmic staining of islet cells.

Specifications

Product Data

• Clone Name: E27
• Applications: IHC, WB
• Recommended Dilution: Western Blot.
  Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/100-1/200
  Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
  Use Polymer anti Mouse/Rabbit IgG as a detection system.
  Positive Control: Pancreas.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified Human Neuron-Specific Enolase (NSE).
• Specificity: Antibody to NSE reacts with gamma isozyme of Human enolase and does not react with alpha isozyme of human enolase.
  Cellular Localization: Cytoplasmic.
• Formulation: State: Supernatant
  State: Liquid Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide.
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens neurofilament, light polypeptide (NEFL)
• Database Link:
  Entrez Gene 4747 HumanEntrez Gene 2026 Human
• Background: Enolases are homo- or heterodimers of the three subunits: alpha (46kDa), beta (44kDa), and gamma (46kDa). The alpha-subunit is expressed in most tissues and the beta-subunit only in muscle. The gammasubunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms. Gamma-Gamma isozyme of enolase is found at elevated concentrations in small cell lung cancer and pediatric neuroblastoma.
• Synonyms: NSE, ENO2, Enolase 2,Neural enolase, Gamma-enolase

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Amyotrophic lateral sclerosis (ALS)