CD11c (ITGAX) Mouse Monoclonal Antibody [Clone ID: 3.9]

CAT#: AM00641BT-N

CD11c (ITGAX) mouse monoclonal antibody, clone 3.9, Biotin

Specifications

Product Data

• Clone Name: 3.9
• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry: We recommend using 1µg to stain 1.0 x 10⁶ cells.
  Immunohistochemistry: Stains macrophages in acetone-fixed frozen section of human lung, brain, colon, skin, spleen, thymus and tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: This antibody recognizes the (Mr 150kDa) alpha chain of the CD11c/CD18 complex.
  Anti-Human CD11c (Clone 3.9) may be used to stain monocytes and macrophages or hairy cell leukemia cells.
• Formulation: 0.01M PBS, pH 7.2
  Label: Biotin
  State: Liquid purified IgG fraction
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
• Concentration: 0.1 mg/ml (OD280nm)
• Purification: Protein G Chromatography
• Conjugation: Biotin
• Database Link:
  Entrez Gene 3687 Human
• Background: CD11c also known as Integrin alpha-X/beta-2 is a receptor for fibrinogen and recognizes the sequence G-P-R in fibrinogen. A member of the integrin alpha chain family, CD11c has seven FG-GAP repeats and a VWFA domain and is a heterodimer of an alpha and a beta subunit where alpha-X associates with beta-2. CD11c binds with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). Recent reports suggest that CD11c helps in capturing Ag in lymphoid tissues and acts as a DC marker, and may even act as an immunotarget for vaccine production and generation of both CD4 and CD8 T-cell responses. The functions of CD11c include phagocytosis of complement coated particles, cell migration, cytokine production by monocytes/macrophages, induction of T cell proliferation by Langerhans cells, leukocyte recruitment, capturing antigens in lymphoid tissues, and the survival of APC. It is predominantly expressed in tissue macrophages, DCs, monocytes and granulocytes.
• Synonyms: ITGAX, Integrin alpha-X, Leu M5