CD22 Mouse Monoclonal Antibody [Clone ID: RFB-4]

CAT#: AM00647RP-N

CD22 mouse monoclonal antibody, clone RFB-4, PE


USD 490.00

2 Weeks*

Size
    • 500 ul

Product Images

Specifications

Product Data
Clone Name RFB-4
Applications IF, IHC
Recommended Dilution

Flow Cytometry: 5 μl is the maximum amount of reagent required for 1 x 106 cells. Lesser amounts of antibody may be sufficient and it is recommended that the customer determine the optimum amount of antibody for each application.
Immunohistochemistry on Frozen Sections: Staining of frozen sections by direct fluorescence or by immuno-chemical techniques.
Quantitative determination of peripheral B-cells (CD22+) in blood.
Identification of CD22-cells in tissue sections.
Determination of B-cell origin of lymphoid neoplasms.
Quantitative elimination or isolation of B-cells by flow cytometry or magnet beads.

Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Specificity This antibody reacts with the CD22 antigen. CD22 is a heterodimer consisting out of 2 glycoproteins (130 and 140 kD). 
Antigen Distribution:
Peripheral blood lymphocytes: 12±3%
T-cells (E+): < 1%
B-cells (E-, Ig+): 92±3%
Monocytes (CD14+): < 1%
Granulocytes: < 1%
Thymocytes: < 1%
Formulation Label: PE
State: Liquid purified IgG fraction
Stabilizer: A highly purified grade of BSA has been added as a stabilizing protein to bring the final protein concentration to 4-5 mg/ml
Preservative: Sodium Azide
Molar radio: F/P :1
Concentration lot specific
Conjugation PE
Storage Store the antibody undiluted in the dark at 2-8°C.
DO NOT FREEZE!
Stability Shelf life: one year from despatch.
Gene Name CD22 molecule
Background CD22 is a heterodimer consisting out of 2 glycoproteins (130 and 140 kD). The molecule is involved in signal transduction in B-cells activated via surface immunoglobulines. CD22 is in principle a B lineage antigen, that is present in the cytoplasm of progenitor B-cells and on the membrane of the majority of mature peripheral B-lymphocytes. Fur-thermore, it is strongly expressed on Hairy Cell Leukemia cells and very weakly on some other leukemias.
Synonyms SIGLEC2, Siglec-2, B-cell receptor CD22, Leu-14, BL-CAM
Reference Data

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.