CD44 Rat Monoclonal Antibody [Clone ID: IM7]

CAT#: AM00699FC-N

CD44 rat monoclonal antibody, clone IM7, FITC

Specifications

Product Data

• Clone Name: IM7
• Applications: FC, IHC, IP
• Recommended Dilution: Flow Cytometry: Use 1 μg to stain 1.0 x 10⁶ cells.
  Immunoprecipitation.
  Immunohistochemistry of acetone fixed frozen tissue sections.
• Reactivities: Canine, Human, Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Specificity: Recognizes all isoforms of the Pgp-1 cell surface glycoprotein. Present on B-cells, monocytes, macrophages and certain subsets of thymocytes and peripheral T-cells. Mice with the Ly-24.1 allotype have high densities of CD44+ T-cells, while Ly-24.2 strains are low expressors.
• Formulation: 0.01M PBS, pH 7.4, 150mM Sodium Chloride
  Label: FITC
  State: Liquid purified IgG fraction from Tissue Culture
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Protein A Chromatography
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 960 Human
• Background: CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
• Synonyms: LHR, MDU2, MDU3, MIC4, CDw44, Epican, ECMR-III, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, PGP-1