Tfrc Rat Monoclonal Antibody [Clone ID: R17217.1.4]

CAT#: AM00702BT-N

Tfrc rat monoclonal antibody, clone R17217.1.4, Biotin


USD 230.00

2 Weeks*

Size
    • 100 ug

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Specifications

Product Data
Clone Name R17217.1.4
Applications FC
Recommended Dilution Flow Cytometry.
Reactivities Mouse
Host Rat
Isotype IgG2a
Clonality Monoclonal
Specificity Anti-mouse CD71 antigen monoclonal antibody reacts with the transferrin receptor (CD71). The expression of CD71 is up-regulated after B cell or T cell activation.
Formulation PBS containing 0.09% sodium azide (NaN3) as preservative and EIA grade BSA as a stabilizing protein
Label: Biotin
State: Liquid purified Ig fraction
Concentration 0,1 mg/ml
Conjugation Biotin
Gene Name Mus musculus transferrin receptor (Tfrc)
Synonyms TfR1, p90, Transferrin receptor protein 1
Note Protocol: FLOW CYTOMETRY ANALYSIS:
Method:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 μl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test).
4. To each tube, add ~1.0 μg of this antibody per 10e6 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C.
7. Wash 2 times at 4°C.
8. Add 100 μl of secondary antibody (Streptavidin-FITC) at a 1/500 dilution.
9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protected from light since most fluorochromes are light sensitive).
10. Wash 2 times at 4°C.
11. Resuspend the cell pellet in 50 μl ice cold media B.
12. Transfer to suitable tubes for flow cytometric analysis containing 15 μl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.
Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 μl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 μl of 2M sodium azide in 100 mls).
Results:
Tissue Distribution by Flow Cytometry Analysis:
(Representative Histogram)
Mouse Strain: Mouse Hybridomas
Cell Concentration: 1x10e6 cells per test
Antibody Concentration Used: 1.0 μg/10e6 cells
Isotypic Control: Biotin Rat IgG2a
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.