E. coli Shiga-like Toxin 1 Mouse Monoclonal Antibody [Clone ID: 286-13310]

CAT#: AM00730PU-N

E. coli Shiga-like Toxin 1 mouse monoclonal antibody, clone 286-13310, Purified

Specifications

Product Data

• Clone Name: 286-13310
• Applications: ELISA, NEUT, WB
• Recommended Dilution: Toxin Neutralization: Neutralized Vero Cell toxicity of E. coli O157:H7 extracts when used at a 10 µg/ml concentration.
  Immunoblot: Detected SLT 1 when used at 1 µg/ml concentration.
  ELISA: Used as capture antibody at 20 µg/ml concentration (100 µl); Immobilizes SLT 1 for detection by an HRP conjugate.
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: SLT Toxin from E. coli O157:H7
• Specificity: This antibody reacts with alpha, alpha-1 and beta subunits of SLT-1 (STX-1). Also reacts with alpha and alpha-1 subunits of SLT-2 (STX-2).
• Formulation: 10 mM Phosphate, pH 7.4 containing 0.9% Sodium chloride.
  State: Purified
  State: Lyophilized purified IgG fraction.
• Reconstitution Method: Restore with 1 ml distilled water
• Concentration: lot specific
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store the antibody undiluted at 2-8°C for two weeks
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
• Synonyms: Escherichia coli, EHEC, EPEC, ETEC