GAPDH Mouse Monoclonal Antibody [Clone ID: 4G5]

CAT#: AM00848PU-N

GAPDH mouse monoclonal antibody, clone 4G5, Purified

Specifications

Product Data

• Clone Name: 4G5
• Applications: ELISA, WB
• Recommended Dilution: ELISA.
  Western blot.
• Reactivities: Bovine, Canine, Feline, Fish, Goat, Human, Mouse, Porcine, Rabbit, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rabbit muscle GAPDH. Hybridization of Sp2/0 myeloma cells with spleen cells from BALB/c mice.
• Specificity: This antibody recognizes GAPDH monomer (36 kDa) and the dimer form.
  Does not react with the tetrameric form.
• Formulation: PBS, pH 7.4 containing 0,09% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction (>90% pure by SDS-PAGE).
• Concentration: lot specific
• Purification: Protein A Chromatography.
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 2597 Human
• Background: Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age-related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed in almost all tissues at high levels, therefore becoming the marker of choice when a loading control in Western blotting is required. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
• Synonyms: GAPD, CDABP0047