Gram Positive Bacteria Mouse Monoclonal Antibody [Clone ID: BDI380]

CAT#: AM00857PU-N

Gram Positive Bacteria mouse monoclonal antibody, clone BDI380, Purified

Specifications

Product Data

• Clone Name: BDI380
• Applications: ELISA, IF, LF
• Recommended Dilution: ELISA.
  IFA.
  Colloidal gold conjugate.
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Intact Listeria monocytogenes.
• Specificity: This antibody clone BDI380 is reactive with lipoteichoic acid (LTA) of many Gram Positive bacteria.
  Cross-reacts with Listeria monocytogenes (all serotypes), Streptococcus pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecium, Bacillus cereus, Bacillus subtilis and group B Streptococcus (weak).
  Does not react with Clostridium perfringens.
• Formulation: 0.01M PBS, pH 7.2
  State: Purified
  State: Liquid purified IgG fraction (>90% pure)
  Stabilizer: None
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Protein A Chromatography
• Storage: Store the antibody undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Bacteria cells are classified as Gram-positive if they retain a crystal violet dye during the Gram stain process. Gram-positive bacteria appear blue or violet under a microscope after the stain has been applied, whereas Gram-negative bacterial look red or pink. This difference in color is mainly due to the characteristics of the cell wall. Gram-positive bacteria generally have a thicker layer of peptidoglycan, a polymer consisting of sugars and amino acids that forms a homogeneous layer outside the plasma membrane. Gram-positive bacteria also have two rings supporting any flagellum and teichoic acids in the cell wall that function as as chelating agents and aid in adherence. Major groups of Gram-positive bacteria include the genera Bacillus, Listeria, Staphylococcus, Streptococcus, Enterococcus and Clostridium, as well as the phylum Actinobacteria. Gram-positive bacteria markers comprise a variety of proteins present on Gram-positive cells, and can aid in the study of function and behavior of this type of bacteria.