CD19 Mouse Monoclonal Antibody [Clone ID: Bu12]

CAT#: AM01058PU-T

CD19 mouse monoclonal antibody, clone Bu12, Purified

Product Images

Staining of human peripheral blood lymphocytes with mouse anti human CD19

Specifications

Product Data

• Clone Name: Bu12
• Applications: FC, IP
• Recommended Dilution: Flow Cytometry: 1/50 - 1/200; 10µl of the suggested working dilution to label 1x10e6 cells in 100µl.
  Immunoprecipitation.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human EB-4 Burkitt lymphoma cell line.
• Specificity: This antibody recognises CD19, a 95kD cell surface glycoprotein, which is expressed on cells of the B cell lineage and follicular dendritic cells but absent on plasma cells. CD19 is an important signal transduction molecule which is involved in the regulation of B lymphocyte development, activation and differentiation.
• Formulation: Phosphate buffered saline pH7.4 containing 0.09% Sodium Azide (NaN3)
  State: Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 930 Human
• Background: CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lamda light chains. It is expressed on approximately 10% of normal human peripheral blood cells and approximately 60% of splenic lymphocytes. It is not expressed on granulocytes, monocytes or T cells as defined by CD3 expression. CD19 defines a pan B antigen which is expressed from the earliest stages of B progenitor development, but is lost on terminal differentiation to plasma cells. It may also be present on some early myeloid progenitors, particularly those of the monoblastic type. The CD19 antigen is expressed on approximately 12% of peripheral blood lymphocytes. It appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen.
• Synonyms: Leu-12, B-cell marker