Glucocorticoid Receptor (NR3C1) Mouse Monoclonal Antibody [Clone ID: 5E4]

CAT#: AM01131FC-N

Glucocorticoid Receptor (NR3C1) mouse monoclonal antibody, clone 5E4, FITC

Specifications

Product Data

• Clone Name: 5E4
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10⁶ cells.
  Membrane permeabilisation is required for this application.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: 26 amino acid peptide corresponding to residues 150-176 on Human GCR linked to thyroglobulin
• Specificity: This antibody recognises a glucocorticoid receptor, located in the cytoplasm of cells and associated with certain heat shock proteins.
• Formulation: PBS, pH 7.4, containing
  Label: FITC
  State: Liquid purified IgG fraction
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Ion Exchange Chromatography
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 2908 Human
• Background: Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor.
• Synonyms: NR3C1, GR, GRL