Dopamine Transporter (SLC6A3) (N-term) Rat Monoclonal Antibody [Clone ID: 4F8]

CAT#: AM01272SU-N

Dopamine Transporter (SLC6A3) (N-term) rat monoclonal antibody, clone 4F8, Supernatant

Specifications

Product Data

• Clone Name: 4F8
• Applications: IHC, WB
• Recommended Dilution: Western Blot: Recognizes a broad band at 70-80 kD. This antibody is not recommended for Western blotting when using rat samples.
  Immunohistochemistry on Frozen Sections: 1/1000-1/10000.
  Incubate the primary antibody, diluted appropriately in TBS with 1% serum, for 2 hours at RT followed by 16 hours at 4°C. A PAP detection system is recommended when working with rat tissue sections; use 4% paraformaldehyde.
  Recommended Positive Control: Brain (caudate, putamen and nucleus accumbens).
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: N-terminus of Human Dopamine transporter fused to Glutathione Stransferase.
• Specificity: This antibody reacts to Dopamine transporter, N-terminus.
• Formulation: State: Supernatant
  State: Liquid Tissue Culture Supernatant
  Stabilizer: None
  Preservative: None
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 6531 Human
• Background: Plasmalemmal neurotransmitter transporters sequester synaptic and peri synaptic transmitter into presynaptic elements. The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. Levels of DAT protein expression are altered by chronic drug administration. The activity of the DAT reuptake carrier is sodium dependent, and it is suspected to play a role in such neurologic and psychiatric disorders as Parkinson's disease, Tourette's disease, schizophrenia, and addiction. It is a 12 transmembrane domain transporter with the N and C terminal regions located within the cytoplasm.
• Synonyms: Sodium-dependent dopamine transporter, DA transporter, DAT1