Pdcd1lg2 Rat Monoclonal Antibody [Clone ID: TY25]

CAT#: AM01313FC-N

Pdcd1lg2 rat monoclonal antibody, clone TY25, FITC

Specifications

Product Data

• Clone Name: TY25
• Applications: FC
• Recommended Dilution: Flow cytometry (Neat - 1/5): use 10 µl of the suggested working dilution to label 10⁶ cells in 100 µl.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: B7-DC transfected RAW264.7 cells. Lymph node cells from immunised SD rats were fused with the cells of the P3U1 myeloma cell line.
• Specificity: This antibody clone TY25 recognizes Mouse CD273.
  This clone TY25 is reported to block the binding of CD273 (B7-DC) to it's receptor (Matsumoto et al. 2004). Removal of sodium azide is recommended prior to use in functional assays.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide and 1% BSA
  Label: FITC
  State: Liquid purified IgG fraction
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.1 mg/ml
• Purification: Affinity chromatography on Protein G
• Conjugation: FITC
• Database Link:
  Entrez Gene 58205 Mouse
• Background: CD273 is a member of the B7 family, otherwise known as B7-dendritic cell (B7-DC), or programmed death ligand-2 (PD-L2).
  The expression of CD273 is restricted to a subpopulation of dendritic cells (DCs), and DCs and macrophages that have been stimulated with interferon gamma, GM-CSF or IL-4. CD273 has been identified as a ligand for PD-1(CD279), along with another B7 family member B7-H1 (CD279). Studies suggest that both CD273 and B7-H1 co-stimulate or inhibit T cell proliferation and cytokine production.
• Synonyms: PD-L2, PD-1-ligand 2, PDCD1 ligand 2, PDCD1LG2, B7DC, PDCD1L2