CD16 (FCGR3A) Mouse Monoclonal Antibody [Clone ID: DJ130c]
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Specifications
Product Data | |
Clone Name | DJ130c |
Applications | FC |
Recommended Dilution | Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 1x106 cells in 100 µl. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | CD34 antigen. |
Specificity | This antibody recognises CD16, a 50-65kD cell surface molecule. DJ130c recognises all polymorphonuclear cells irrespective on their NA phenotype. Studies show that the DJ130c antibody recognises an epitope in the first membrane-distal domain of CD16 and recognises both CD16A and CD16B. |
Formulation | PBS, pH 7.4 Label: Biotin State: Liquid purified IgG fraction from Tissue Culture Supernatant Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein G |
Conjugation | Biotin |
Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Database Link | |
Background | CD16 is the low affinity receptor for IgG (FcR III). CD16 exists as a transmembranous form (Fc gammaRIIIA, or CD16A) and a glycosyl phosphatidylinositol (GPI) anchored form (Fc gammaRIIIB, or CD16B). CD16A is expressed by NK cells, some T cells, and macrophages, whereas CD16B is primarily expressed by granulocytes. In addition, CD16B exists as two allelic variants NA1 and NA2. |
Synonyms | FCGR3A, CD16A, FCG3, FCGR3, IGFR3, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2 |
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