CD16 (FCGR3A) Mouse Monoclonal Antibody [Clone ID: DJ130c]

CAT#: AM01321PU-T

CD16 (FCGR3A) mouse monoclonal antibody, clone DJ130c, Purified


USD 190.00

5 Days*

Size
    • 25 ug

Product Images

Specifications

Product Data
Clone Name DJ130c
Applications FC, IHC
Recommended Dilution Flow Cytometry: Use 10 µl of 1/20-1/200 diluted antibody to label 1x106 cells in 100 µl.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. 
Recommended Positive Control: Human tonsil.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen CD34 antigen
Specificity This antibody recognizes Human CD16, a 50-65kD cell surface molecule. It recognizes all polymorphonuclear cells irrespective on their NA phenotype.
Studies show that the DJ130c antibody recognizes an epitope in the first membrane-distal domain of CD16 and recognizes both CD16a and CD16b and has been demonstrated to cross-react with CD16 from Rhesus macaques, Macaca mulatta (Xu et al. 2012).
Formulation PBS
State: Purified
State: Liquid purified IgG fraction from Tissue Culture Supernatant
Preservative: 0.09% Sodium Azide
Concentration lot specific
Purification Affinity Chromatography on Protein G
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background CD16 which is the low affinity receptor for IgG (FcRIII), exists as a transmembranous form (Fc gammaRIIIA, or CD16a) and a glycosyl phosphatidylinositol (GPI) anchored form (Fc gammaRIIIB, or CD16b). CD16a is expressed by NK cells, some T cells, and macrophages, whereas CD16b is primarily expressed by granulocytes. In addition, CD16b exists as two allelic variants NA1 and NA2.
Synonyms FCGR3A, CD16A, FCG3, FCGR3, IGFR3, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.