CD16 (FCGR3A) Mouse Monoclonal Antibody [Clone ID: DJ130c]

CAT#: AM01321RP-N

CD16 (FCGR3A) mouse monoclonal antibody, clone DJ130c, PE

Specifications

Product Data

• Clone Name: DJ130c
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 10⁶ cells in 100 µl.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: CD34 antigen
• Specificity: This antibody recognizes CD16, a 50-65kD cell surface molecule.
  Studies show that the CD16 antibody Clone DJ130c recognizes an epitope in the first membrane-distal domain of CD16 and recognizes both CD16a and CD16b and has been demonstrated to cross-react with CD16 from rhesus macaques, Macaca mulatta (Xu et al. 2012).
• Formulation: PBS, pH 7.4
  Label: PE
  State: Lyophilized purified IgG fraction
  Stabilizer: 1% BSA, 5% Sucrose
  Preservative: 0.09% Sodium Azide
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with 1.0 ml distilled water
• Purification: Affinity Chromatography on Protein G
• Conjugation: PE
• Storage: Prior to and following reconstitution store the antibody at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 2214 Human
• Background: CD16 is the low affinity receptor for IgG (FcR III). CD16 exists as a transmembranous form (Fc gammaRIIIA, or CD16A) and a glycosyl phosphatidylinositol (GPI) anchored form (Fc gammaRIIIB, or CD16B). CD16A is expressed by NK cells, some T cells, and macrophages, whereas CD16B is primarily expressed by granulocytes. In addition, CD16B exists as two allelic variants NA1 and NA2. DJ130c recognises all polymorphonuclear cells irrespective on their NA phenotype.
• Synonyms: FCGR3A, CD16A, FCG3, FCGR3, IGFR3, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2