CD79A (202-216) Mouse Monoclonal Antibody [Clone ID: HM57]
Specifications
Product Data | |
Clone Name | HM57 |
Applications | FC, IHC, WB |
Recommended Dilution | Western blot. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/100-1/500. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Human tonsil. Flow Cytometry: Use 10 µl of 1/10-1/50 diluted CD79a antibody to label 1x106 cells in 100 µl (Membrane permeabilisation is required). |
Reactivities | Bison, Bovine, Canine, Chicken, Deer, Equine, Guinea Pig, Human, Monkey, Mouse, Porcine, Rabbit, Rat |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Synthetic peptide corresponding to 202-216 amino acid sequence of Human mb-1. |
Specificity | This antibody recognizes an epitope within the cytoplasmic domain of CD79a. CD79a, also known as mb-1, is a 45kD protein that is expressed by B lymphocytes during differentiation from early pre-B cell stage through to plasma cells. |
Formulation | PBS State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein G |
Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Database Link | |
Background | The CD79a molecule associates with CD79b (B29) to form a heterodimer that is non-covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex. The CD79a/CD79b heterodimers are also necessary for intracellular signalling following antigen-binding to surface immunoglobulin. |
Synonyms | IGA, MB1, B-Cell marker |
Reference Data |
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