Complement C3 (C3) Mouse Monoclonal Antibody [Clone ID: 10-02A]

CAT#: AM01326PU-N

Complement C3 (C3) mouse monoclonal antibody, clone 10-02A, Purified

Specifications

Product Data

• Clone Name: 10-02A
• Applications: ELISA, FC, IHC, WB
• Recommended Dilution: ELISA: This antibody binds both C3c and C3 and so is not suitable for all ELISA applications.
  Western Blot.
  Flow Cytometry.
  Immunohistochemistry on frozen sections.
  Recommended Positive Control: Kidney from post streptococal glomerulonephritis patients.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Native human C3 from normal serum, highly purified by chromatography.
• Specificity: This antibody recognises 135kDa complement component 3c (C3c), present in blood serum.
• Formulation: Borate buffered saline pH 8.4 containing 0.02% Sodium Azide
  State: Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens complement component 3 (C3)
• Database Link:
  Entrez Gene 718 Human
• Background: C3c is generated over the course of complement activation, where convertase C4b2a (classical pathway) and convertase C3bBb (alternative pathway) cleave C3 to C3b and C3a. C3b is further degraded into iC3b and C3dg/C3d. iC3b is then slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha chain fragment 1 + alpha chain fragment 2), C3dg and C3f. C3 is crucial in the induction of tolerance generated when an antigen is introduced into immunoprivileged sites and this is exploited by pathogens and cancer cells to evade the immune system by inhibiting complement activation.
• Synonyms: CPAMD1, Complement component 3

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Complement and coagulation cascades, Systemic lupus erythematosus