Complement C4A (C4A) Mouse Monoclonal Antibody [Clone ID: 10-12]

CAT#: AM01328PU-N

Complement C4A (C4A) mouse monoclonal antibody, clone 10-12, Purified

Specifications

Product Data

• Clone Name: 10-12
• Applications: ELISA, FC, IHC, WB
• Recommended Dilution: Western Blot.
  Flow Cytometry.
  ELISA: This antibody binds both C4c and C4 and so is not suitable for all ELISA applications.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections: 10 µg/ml.
  Recommended Postive Control: Kidney sections from post-streptococal glomerulonephritis pastients.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified Human Complement C4c.
• Specificity: This antibody recognises complement component 4c (C4c).
• Formulation: Borate buffered saline, pH 8.4
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens complement component 4A (Rodgers blood group) (C4A), transcript variant 1
• Database Link:
  Entrez Gene 720 Human
• Background: The complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4bs alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonisation and activation of the classical pathway. iC4b is further degraded into the inactive fragments C4d and C4c. C4c consists of two a-chain fragments, alpha 4 (13 kDa) and alpha 3 (25 kDa) linked to a beta chain through disulphide bridges and a gamma chain. It is expressed in the blood plasma.
• Synonyms: Complement Component 4, CPAMD2, CPAMD3

Reference Data

• Protein Pathways: Complement and coagulation cascades, Systemic lupus erythematosus