Complement C5 (C5) Mouse Monoclonal Antibody [Clone ID: 10-04]

CAT#: AM01330PU-N

Complement C5 (C5) mouse monoclonal antibody, clone 10-04, Purified

Specifications

Product Data

• Clone Name: 10-04
• Applications: ELISA, FN, WB
• Recommended Dilution: ELISA.
  Western Blot.
  Functional Assays.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified Human C5
• Specificity: This antibody recognizes secreted complement component 5 (C5), a 190kDa serine protease present in blood serum.
  In haemolysis inhibition studies it was found that this antibody will block C5 haemolytic activity. Removal of Sodium Azide is recommended prior to use in Functional Assays.
• Formulation: Borate buffered saline
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens complement C5 (C5), transcript variant 1
• Database Link:
  Entrez Gene 727 Human
• Background: It is composed of an 115kDa disulphide linked alpha-chain and a 75 kDa beta-chain. In addition to its role in complement, C5 is thought to be associated with inflammation, stimulating tumour necrosis factor alpha, interferon gamma mediated suppression of IL10 and IL12 production. C5 is activated via either the classical or the alternative complement pathway, by proteolytic cleavage of C5a from the N-terminal of the alpha-chain, by C5 convertase. C5a is an anaphylotoxin which mediates local inflammation. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. It also stimulates chemotaxis of neutrophils, eosinophils, basophils and monocytes towards the site of inflammation. The remaining fragment, C5b, initiates the assembly of the membrane attack complex.
• Synonyms: CPAMD4, Complement component 5

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus