Complement factor B (CFB) (Ba Fragment) Mouse Monoclonal Antibody [Clone ID: 014III-33.2.4.3]

CAT#: AM01348PU-N

Complement factor B (CFB) (Ba Fragment) mouse monoclonal antibody, clone 014III-33.2.4.3, Purified

Specifications

Product Data

• Clone Name: 014III-33.2.4.3
• Applications: ELISA, FC, FN, IHC, WB
• Recommended Dilution: Western Blot.
  Functional Assays.
  Flow Cytometry.
  ELISA: This antibody binds both the Ba fragment and the whole protein so is not suitable for all ELISA applications.
  Immunohistochemistry on frozen sections.
  Recommended Positiv Control: Kidney from post streptoccal glomerulonephritis patients.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified human complement factor b.
• Specificity: This antibody recognises the 30kDa Ba fragment of 90kDa complement factor B, present in blood serum.
• Formulation: Borate buffered saline pH 8.2 - 8.4 containing 0.02% Sodium Azide
  State: Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens complement factor B (CFB)
• Database Link:
  Entrez Gene 629 Human
• Background: C3b associates with complement factor B, inducing conformational change. This enables complement factor D to cleave the N-terminal of complement factor B (the Ba subunit), leaving the 63 kDa Bb subunit associated with C3b, forming C3 convertase. Subunit Ba inhibits lymphocyte proliferation. Conversely, subunit Bb is involved in the proliferation of preactivated B lymphocytes.
• Synonyms: Properdin factor B, C3/C5 convertase, PBF2, CFB, BF, BFD
• Note: This antibody inhibits the function of factor B and binds both nascent factor B and the Ba cleavage product. Removal of Sodium Azide is recommended prior to use in functional assays.

Reference Data

• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades