Complement C3 (C3) Mouse Monoclonal Antibody [Clone ID: 053A-514.3.1.4]

CAT#: AM01349PU-N

Complement C3 (C3) mouse monoclonal antibody, clone 053A-514.3.1.4, Purified

Specifications

Product Data

• Clone Name: 053A-514.3.1.4
• Applications: ELISA, FC, IHC
• Recommended Dilution: ELISA.
  Flow Cytometry.
  Immunohistochemistry on Frozen Sections.
  Recommended Positive Control: Kidney from post streptococcal glomerulonephritis patients.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified Human C3d (Neoantigen).
• Specificity: This antibody is specific for complement component 3d (C3d) neoantigen, a 33kDa polypeptide fragment generated over the course of complement activation in which where complement 3 (C3) convertases cleave C3 to C3b, which is further degraded into iC3b and C3dg/C3d.
• Formulation: Borate buffered saline pH 8.2-8.4
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens complement component 3 (C3)
• Database Link:
  Entrez Gene 718 Human
• Background: C3d is involved in the regulation of many aspects of the immune response, including antigen processing and presentation. The C3d/antigen complex mediates B cell activation by simultaneously binding antigen-specific surface bound immunoglobulin and CD21, lowering the complement activation threshold. C3 is crucial in the induction of tolerance generated when an antigen is introduced into immunoprivileged sites and this is exploited by pathogens and cancer cells to evade the immune system by inhibiting complement activation.
• Synonyms: CPAMD1, Complement component 3

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Complement and coagulation cascades, Systemic lupus erythematosus