IL12A Mouse Monoclonal Antibody

CAT#: AM01362PU-N

IL12A mouse monoclonal antibody, Azide Free

Product Images

Immunohistochemistry using Interleukin-12 alpha / IL12A Antibody Cat.-No AM01362PU-N

Specifications

Product Data

• Applications: ELISA, IF, WB
• Recommended Dilution: ELISA: In a Sandwich ELISA (assuming 100 µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant Human IL-12 when used with Biotin anti-Human IL-12 antibody (Cat.-No PP1102B1 or PP1102B2) as the detection antibody at a concentration of at least 0.25-0.5 µg/ml.
  Western Blot: To detect Human IL-12 by Western Blot analysis this antibody can be used at a concentration of 0.50-1.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-12 is 0.5-1.0 ng/lane, under non-reducing conditions.
  Immunohistochemistry: This antibody stained PBMC. The primary antibody was incubated at 10.0 µg/ml for one hour at room temperature followed by a fluorescent labeled secondary antibody.
  Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: CHO cells derived Highly pure (>98%) 75.0 kDa Recombinant Human IL-12 (Cat.-No PA086).
• Specificity: This antibody detects Human Interleukin-12.
  Other species not tested.
• Formulation: PBS without preservatives
  State: Azide Free
  State: Lyophilized (sterile filtered) IgG fraction from Ascites
• Reconstitution Method: Restore in sterile water to a concentration of 1.0 mg/ml.
• Purification: Affinity Chromatography on Protein A
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens interleukin 12A (IL12A)
• Database Link:
  Entrez Gene 3592 Human
• Background: IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-γ production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue type mast cells. Among its biological activities IL-12 promotes the growth and activity of activated NK, CD4⁺ and CD8⁺ cells and induces the development of IFN-Gamma producing Th1 cells.
• Synonyms: IL-12A, IL-12 subunit p35, NKSF1, CLMF p35

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Allograft rejection, Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus