DEFB4B (4-41) Mouse Monoclonal Antibody [Clone ID: L12-4C-C2]

CAT#: AM02221PU-N

DEFB4B (4-41) mouse monoclonal antibody, clone L12-4C-C2, Purified

Product Images

Immunohistochemical staining of Beta-Defensin-2 in Paraffin Section of Human colon resection tissue. Antigen retrieval was performed in citric acid (0.01M, pH6) twice for 7 min and in 0.1% Trypsin for 5 min. The section was incubated with AM02221PU (1/2000) for 1 h and detected using Alkaline Phophatase anti-Alkaline Phophatase method. New fuchsin was used as the chromogen. The section was counterstained with hematoxylin. AM02221PU stains colonic epithelial enterocytes in ulcerative colitis patients. Langhorst J et al. (2009) Am. J. Gastroenterol 104 (2): 404-410.

Specifications

Product Data

• Clone Name: L12-4C-C2
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Western Blot: 5 µg/ml (See Ref.1).
  Immunohistochemistry on Paraffin Sections: 1/2000 (See Ref.1).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide corresponding to amino acids 4-41 of Human beta-Defensin 2
• Specificity: This antibody recognizes Human beta-Defensin 2 (aa 4-41).
  Other species not tested.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Lyophilized purified IgG fraction from Cell Culture Supernatant
• Reconstitution Method: AM02221PU-N: Restore in 0.1 ml aqua bidest to a concentration of 1 mg/ml.
  AM02221PU-S: Restore in 10 µl aqua bidest to a concentration of 1 mg/ml.
• Purification: Protein G Chromatography
• Database Link:
  Entrez Gene 100289462 Human
• Background: This antibiotic peptide is locally regulated by inflammation. Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence.
• Synonyms: Beta-defensin 4A, Beta-defensin 2, BD-2, hBD-2, DEFB102, DEFB2, Skin-antimicrobial peptide 1, DEFB4B, DEFB4A
• Note: LocusID 1673