beta Defensin 3 (DEFB103A) (6-22) Mouse Monoclonal Antibody [Clone ID: L3-18b-E1]

CAT#: AM02222PU-S

beta Defensin 3 (DEFB103A) (6-22) mouse monoclonal antibody, clone L3-18b-E1, Purified

Product Images

Immunohistochemistry of beta-Defensin-3 staining in Paraffin section of Rat lung tissue. Antigen retrieval was performed in 0.01M Sodium Citrate buffer at 55-58°C for 5min. The sections were incubated with AM02222PU (1/50) overnight and detected using a biotinylated secondary antibody in combination with biotin-streptavidin method. DAB was used as substrate. Sections were counterstained with hematoxylin. AM02222PU stains the bronchiolar epithelium in methicillin-resistant Staphylococcus aureus inoculated rats. Original magnefication: x200

Specifications

Product Data

• Clone Name: L3-18b-E1
• Applications: ELISA, IHC, R, WB
• Recommended Dilution: RIA.
  ELISA.
  Western Blot (Ref.1).
  Immunohistochemistry Paraffin Sections: 1/50 (Ref.1).
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic Human beta-Defensin 3 (aa 6-22)
• Specificity: This antibody detects Human beta-Defensin 3 (aa 6-22), Rat beta-Defensin 3.
• Formulation: 50 mM TRIS pH 7.4
  State: Purified
  State: Lyophilized purified IgG fraction from Cell Culture Supernatant
• Reconstitution Method: Restore in aqua bidest to 1 mg/ml.
• Purification: Protein G Chromatography
• Database Link:
  Entrez Gene 414325 Human
• Background: Beta Defensin 3 exhibits antimicrobial activity against Gram positive bacteria S.aureus and S.pyogenes, Gram negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. It kills multiresistant S.aureus and vancomycin resistant E.faecium, but it appears to have no significant hemolytic activity. Beta Defensin 3 is highly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. Low expression is observed in salivary gland, bone marrow, colon, stomach, polyp and larynx, with no expression in small intestine. Expression is induced by infection of bacteria and by interferon gamma. Beta Defensin 3 belongs to the beta defensin family.
• Synonyms: DEFB103A, DEFB103B, BD3, DEFB3, HBP3, HBD3, DEFB103, Beta-defensin 103, Defensin-like protein, Defensin beta 103
• Note: GenID: 55894 and 414325