CD45 (PTPRC) Mouse Monoclonal Antibody [Clone ID: L12/201]

CAT#: AM02304PU-S

CD45 (PTPRC) mouse monoclonal antibody, clone L12/201, Aff - Purified

Specifications

Product Data

• Clone Name: L12/201
• Applications: FC, IF, IHC
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/50-1/100 dilution to label 10⁶ cells or 100µl whole blood.
  Immunohistochemistry on Frozen Sections.
  Immunofluorescence.
  Immunoprecipitation was only obtained after cross linking antibody to the labelled cell surface. This gave a band of 200 kDa.
• Reactivities: Rabbit
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Glycoproteins isolated from the T cell line, RL-5.
  Spleen cells from immunised mice were fused with cells of the P3.X63.Ag8-U1 mouse myeloma cell line.
• Specificity: This antibody shows pan leucocyte reactivity by flow cytometry and immunohistochemistry.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200