VCP Mouse Monoclonal Antibody [Clone ID: Hs-14]

CAT#: AM03029FC-N

VCP mouse monoclonal antibody, clone Hs-14, FITC


USD 355.00

2 Weeks*

Size
    • 50 Tests

Product Images

Specifications

Product Data
Clone Name Hs-14
Applications FC, IF
Recommended Dilution Suitable for Flow Cytometry analysis of sperm cells using 20 μl reagent / 100 μl cell suspension.
The content of a vial (1 ml) is sufficient for 50 tests.
Reactivities Human, Mouse
Host Mouse
Isotype IgM
Clonality Monoclonal
Immunogen Freshly ejaculated Human sperms were washed in PBS and extracted in 3% Acetic Acid, 10% Glycerol, 30mM benzaminidine. The acid extract was dialyzed against 0.2% Acetic Acid and subsequently used for immunization.
Specificity The antibody  reacts with VCP (valosin-containing protein) a 220 kDa protein previously identified under the general name "intra-acrosomal protein".
Formulation Tris buffered saline (TBS) containing 15 mM Sodium Azide as preservative and 0.2% (w/v) high-grade BSA (protease free) as stabilizer
Label: FITC
State: Liquid purified Ig fraction
Label: Conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
Conjugation FITC
Storage Store the antibody in the dark at 2-8°C.
DO NOT FREEZE!
Avoid prolonged exposure to light.
Stability Shelf life: One year from despatch.
Background VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
Synonyms 15S Mg(2+)-ATPase p97 subunit, Valosin-containing protein
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.