VCP Mouse Monoclonal Antibody [Clone ID: Hs-14]

CAT#: AM03029FC-N

VCP mouse monoclonal antibody, clone Hs-14, FITC

Product Images

Figure 1. Immunofluorescence analysis of 220 kDa intra-acrosomal protein in Acetone-permeabilized Human sperms using monoclonal antibody [AM03029] Clone Hs-14 (normal spermiogram).

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  Staining of VCP in acrosomes of human sperms using monoclonal antibody Hs-14 FITC.

Specifications

Product Data

• Clone Name: Hs-14
• Applications: FC, IF
• Recommended Dilution: Suitable for Flow Cytometry analysis of sperm cells using 20 μl reagent / 100 μl cell suspension.
  The content of a vial (1 ml) is sufficient for 50 tests.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Freshly ejaculated Human sperms were washed in PBS and extracted in 3% Acetic Acid, 10% Glycerol, 30mM benzaminidine. The acid extract was dialyzed against 0.2% Acetic Acid and subsequently used for immunization.
• Specificity: The antibody reacts with VCP (valosin-containing protein) a 220 kDa protein previously identified under the general name "intra-acrosomal protein".
• Formulation: Tris buffered saline (TBS) containing 15 mM Sodium Azide as preservative and 0.2% (w/v) high-grade BSA (protease free) as stabilizer
  Label: FITC
  State: Liquid purified Ig fraction
  Label: Conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
• Conjugation: FITC
• Storage: Store the antibody in the dark at 2-8°C.
  DO NOT FREEZE!
  Avoid prolonged exposure to light.
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 7415 Human
• Background: VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
• Synonyms: 15S Mg(2+)-ATPase p97 subunit, Valosin-containing protein