Human Kappa Light Chain Mouse Monoclonal Antibody [Clone ID: A8B5]

CAT#: AM03059FC-N

Human Kappa Light Chain mouse monoclonal antibody, clone A8B5, FITC

Product Images

B lymphocytes (CD19+) in a dot-plot Lambda PE vs. Kappa FITC. Kappa light chain detected by A8B5 antibody and lambda light chain by 4C2 antibody, CD19 by LT19 antibody.

Specifications

Product Data

• Clone Name: A8B5
• Applications: FC
• Recommended Dilution: Flow Cytometry analysis of Human blood cells using 20 µl reagent/100 µl of whole blood or 10⁶ cells in a suspension.
  The content of a vial (2 ml) is sufficient for 100 tests.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: The antibody A8B5 reacts with Human kappa light chains (22.5 kDa) of immunoglobulins.
  Negative Species: Hamster, Sheep, Goat, Rabbit and Guinea pig.
• Formulation: Phosphate buffered saline (PBS) solution containing 15mM sodium azide
  Label: FITC
  State: Liquid purified Ig fraction
  Label: Conjugated with Fluorescein isothiocyanate under optimum conditions. The reagent is free of unconjugated and adjusted for direct use.
• Conjugation: FITC
• Background: Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.