CD95 (FAS) Mouse Monoclonal Antibody [Clone ID: LT95]

CAT#: AM03061FC-N

CD95 (FAS) mouse monoclonal antibody, clone LT95, FITC

Specifications

Product Data

• Clone Name: LT95
• Applications: FC
• Recommended Dilution: This antibody is suitable for Flow Cytometry analysis of human blood cells using 20 μl reagent/100 μl of whole blood or 10e6 cells in a suspension.
  The content of a vial (2 ml) is sufficient for 100 tests.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: HUT-78 human T cell lymphoma cell line
• Specificity: The antibody LT95 reacts with CD95 (Fas/APO-1), a 46 kDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells.
  It seems that the antibody LT95 does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule.
• Formulation: PBS containing 15 mM sodium azide as preservative and 0.2% (w/v) high-grade BSA (Protease free) as stabilizer.
  Label: FITC
  State: Liquid purified IgG fraction.
  Label: Conjugated with Fluorescein isothiocyanate under optimum conditions
• Conjugation: FITC
• Storage: Store the antibody in the dark at 2-8°C.
  Do Not Freeze!
  Avoid prolonged exposure to light.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 355 Human
• Background: CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.
• Synonyms: FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6