CD95 (FAS) Mouse Monoclonal Antibody [Clone ID: LT95]

CAT#: AM03061PU-N

CD95 (FAS) mouse monoclonal antibody, clone LT95, Purified

Product Images

Flow cytometry analysis of Fas (CD95) expression on the surface of various hematopoietic cell lines with purified anti-human CD95 (LT95) (detection by Goat anti-mouse IgG1 PE). Histograms: Red -Isotype mouse IgG1 control staining Black --standard anti--CD95 monoclonal antibody (DX2) Green --anti-CD95 (LT95) (A -JURKAT human peripheral blood T cell leukemia cell line; B -RAMOS human Burkitt lymphoma cell line; C -CEM human leukemia cell line; D -MOLT-4 human acute lymphoblastic T cell leukemia cell line)

Specifications

Product Data

• Clone Name: LT95
• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry.
  Immunohistochemistry on Paraffin Sections.
  Positive tissue: tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: HUT-78 Human T cell lymphoma cell line.
• Specificity: The antibody LT95 reacts with CD95 (Fas/APO-1), a 46 kDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells.
  It seems that the antibody LT95 does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Concentration: lot specific
• Purification: Protein-A Affinity Chromatography
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 355 Human
• Background: CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.
• Synonyms: FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6