CD95 (FAS) Mouse Monoclonal Antibody [Clone ID: LT95]

CAT#: AM03061RP-N

CD95 (FAS) mouse monoclonal antibody, clone LT95, PE

Product Images

Surface staining of human peripheral blood cells with anti-CD95 (LT95) PE.

Specifications

Product Data

• Clone Name: LT95
• Applications: FC
• Recommended Dilution: Flow Cytometry: use 20 μl reagent / 100 μl of whole blood or 10e6 cells in a suspension.
  The content of a vial (2 ml) is sufficient for 100 tests.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: HUT-78 human T cell lymphoma cell line.
• Specificity: The antibody LT95 reacts with CD95 (Fas/APO-1), a 46 kDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells.
  It seems that the antibody LT95 does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule.
• Formulation: Phosphate buffered saline (PBS)
  Label: PE
  State: Liquid purified IgG fraction.
  Stabilizer: 0.2% (w/v) high-grade BSA (Protease free)
  Preservative: 15 mM sodium azide
  Label: Conjugated with R-Phycoerythrin under optimum conditions
• Purification: Size-exclusion chromatography.
• Conjugation: PE
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 355 Human
• Background: CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.
• Synonyms: FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6