HLAG (HLA-G) (C-term) Mouse Monoclonal Antibody [Clone ID: 2A12]

CAT#: AM03082PU-N

HLAG (HLA-G) (C-term) mouse monoclonal antibody, clone 2A12, Purified

Product Images

Surface staining of HLA-G (on transfected cells) with anti-HLA-G (2A12) purified, GAM-APC.

Specifications

Product Data

• Clone Name: 2A12
• Applications: FC, IF, IHC, WB
• Recommended Dilution: Flow Cytometry: excellent for intracellular staining; 1-10 µg/ml.
  Western Blot.
  Immunohistochemistry (paraffin and frozen sections).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin
• Specificity: The antibody 2A12 allows to discriminate between soluble HLA-G protein yielded by shedding from membrane-bound HLA-G forms that do not contain the intron 4-encoded epitope and soluble HLA-G5/HLA-G6 produced from intron 4-retaining alternatively spliced mRNAs. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
• Formulation: PBS, pH 7.4 with 15 mM sodium azide as preservative
  State: Purified
  State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE)
• Concentration: 1.0 mg/ml
• Purification: Purified by protein A
• Database Link:
  Entrez Gene 3135 Human
• Background: HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternate splicing provides both soluble and membrane bound forms of HLA G, with two soluble isoforms and four membrane bound isoforms. HLA G may be part of the fetal protection system and is expressed on fetal derived placental cells.
• Synonyms: HLA-6.0, HLAG, MHC class I antigen G