HLAG (HLA-G) Mouse Monoclonal Antibody [Clone ID: 01G]

CAT#: AM03084PU-N

HLAG (HLA-G) mouse monoclonal antibody, clone 01G, Purified

Product Images

Surface staining of HLA-G transfected LCL cells with anti-HLA-G (01G) purified, GAM-APC.

Specifications

Product Data

• Clone Name: 01G
• Applications: ELISA, FC, IF, IHC, IP
• Recommended Dilution: Flow Cytometry: 5-6 µg/ml.
  Immunoprecipitation.
  Immunohistochemistry (frozen sections).
  Immunocytochemistry.
  ELISA.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.
• Specificity: The antibody 01G recognizes membrane-bound form of HLA-G (full-length HLA-G1), but not soluble forms. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
• Formulation: PBS, pH 7.4 with 15 mM sodium azide as preservative
  State: Purified
  State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE)
• Concentration: 1.0 mg/ml
• Purification: Protein A affinity chromatography
• Database Link:
  Entrez Gene 3135 Human
• Background: HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternate splicing provides both soluble and membrane bound forms of HLA G, with two soluble isoforms and four membrane bound isoforms. HLA G may be part of the fetal protection system and is expressed on fetal derived placental cells.
• Synonyms: HLA-6.0, HLAG, MHC class I antigen G