CD30 (TNFRSF8) Mouse Monoclonal Antibody [Clone ID: MEM-268]

CAT#: AM03094PU-N

CD30 (TNFRSF8) mouse monoclonal antibody, clone MEM-268, Purified

Product Images

Surface staining of K562 cells with anti-human CD30 (MEM-268) purified, GAM APC.

Specifications

Product Data

• Clone Name: MEM-268
• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry: 5 µg/ml.
  Immunohistochemistry on Paraffin Sections (5 μg/ml).
  Positive tissue: thymus.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Expression vector containing CD30 cDNA (booster suspension of THP-1 cell line)
• Specificity: The antibody MEM-268 recognizes extracellular part of CD30 (Ki-1 antigen), a 105 kDa single chain glycoprotein expressed on Hodgkin's and Reed-Sternberg cells; it is also found in Burkitt's lymphomas, virus-infected T and B lymphocytes, and on normal B and T lymphocytes after activation (T lymphocytes that produce Th2-type cytokines and on CD4+/CD8+ T lymphocytes that co-express CD45RO and the IL4 receptor).
• Formulation: Phosphate buffered saline (PBS), pH 7.4 containing 15 mM Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 943 Human
• Background: CD30 is a type I transmembrane glycoprotein of the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkins and Reed-Sternberg cells using monoclonal antibody Ki-1. The ligand for CD30 is CD30L (CD153). The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. CD30 has a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. In addition to its expression on Hodgkin's and Reed-Sternberg cells, CD30 is also found in some non-Hodgkin's lymphomas (including Burkitt's lymphomas), virus-infected T and B cells, and on normal T and B cells after activation. In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that co-express CD45RO and the IL4 receptor. Soluble form of CD30 (sCD30) serves as a marker reflecting Th2 immune response.
• Synonyms: TNFRSF8, D1S166E, CD30L receptor, KI-1 antigen