CD5 Mouse Monoclonal Antibody [Clone ID: CRIS1]

CAT#: AM03103PU-N

CD5 mouse monoclonal antibody, clone CRIS1, Aff - Purified


USD 220.00

2 Weeks*

Size
    • 100 ug

Product Images

Specifications

Product Data
Clone Name CRIS1
Applications ELISA, FC, IHC, IP, WB
Recommended Dilution Immunoprecipitation.
Flow cytometry: 1 μg/ml
Positive Control:
Peripheral Blood Lymphocytes (PBL)
Jurkat human leukemia T-cell line.
HPB human leukemia T-cell line.
MOLT-4 human leukemia T-cell line.
Western blot: 1-2 μg/ml
Positive Control:
Jurkat human leukemia T-cell line.
HPB human leukemia T-cell line.
Immunohistochemistry (Frozen sections).
ELISA:
Can be used in the Sandwich ELISA as the detection antibody in pair with the capture antibody MEM-32.
Reactivities Human
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Stimulated human leukocytes.
Specificity The antibody CRIS1 reacts with the cell surface glycoprotein CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).
Formulation PBS, pH 7.4 with 15 mM sodium azide as preservative.
State: Aff - Purified
State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE).
Concentration 1.0 mg/ml
Purification Affinity Chromatography on Protein-A.
Gene Name CD5 molecule
Background CD5 antigen (T1; 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies.
Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (Rheumatoid Arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.
Synonyms CD5, LEU1
Reference Data

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