Clathrin heavy chain (CLTC) Mouse Monoclonal Antibody [Clone ID: BF-06]

CAT#: AM03115PU-N

Clathrin heavy chain (CLTC) mouse monoclonal antibody, clone BF-06, Purified

Product Images

Immunofluorescence staining of clathrin in human primary fibroblasts using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

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  Immunofluorescence staining of clathrin in human HeLa cell line using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Specifications

Product Data

• Clone Name: BF-06
• Applications: ELISA, FC, IF, IP, WB
• Recommended Dilution: Flow Cytometry: 2-5 µg/ml.
  Positive control: Human blood leukocytes.
  Immunoprecipitation.
  Western Blotting.
  Immunocytochemistry.
  ELISA.
• Reactivities: Bovine, Human, Mouse, Porcine, Rat
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Specificity: The antibody recognizes Clathrin heavy chain, an ubiquitously expressed 180 kDa protein involved in receptor-mediated endocytosis.
• Formulation: Stabilising Tris buffered saline (TBS) with 15 mM Sodium Azide, approx. pH 8.0
  State: Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
• Concentration: lot specific
• Purification: Precipitation and Chromatography
• Storage: Store the antibody at 2-8°C.
  Do not freeze.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens clathrin heavy chain (CLTC), transcript variant 1
• Database Link:
  Entrez Gene 54241 RatEntrez Gene 67300 MouseEntrez Gene 1213 Human
• Background: Clathrin is a submembrane protein that polymerizes into coat-like lattices, which results in membrane invagination. The basic oligomers are composed of three clathrin heavy chain (180 kDa) and three light chain (30 kDa) subunits and the process of polymerization is dynamically regulated by the light chains. Interaction of clathrin with the plasma membrane is mediated by adaptor proteins (AP1-4) specific for different cellular compartments. Another proteins, such as endophilin, epsin and amphiphysin are involved in membrane invagination and clathrin rearrangements. Finally, dynamin functions at the fission stage of clathrin-mediated endocytosis.
• Synonyms: CLTC, CLH17, CLTCL2, KIAA0034, Membrane Vesicle Marker

Reference Data

• Protein Pathways: Endocytosis, Huntington's disease, Lysosome