CD69 Mouse Monoclonal Antibody [Clone ID: FN50]

CAT#: AM03132PU-N

CD69 mouse monoclonal antibody, clone FN50, Aff - Purified

Product Images

Surface staining of Human peripheral blood using anti-CD69 antibody (clone FN50) after overnight activation of T cells by anti-CD3 (clone MEM-57).

Specifications

Product Data

• Clone Name: FN50
• Applications: FC, FN, IHC, WB
• Recommended Dilution: Flow Cytometry: 5 µg/ml.
  Western blot: 53 kDa, under reducing conditions it shows two bands of 29 and 32 kDa.
  Immunohistochemistry on Frozen Sections.
  Functional Assay: When stimulating peripheral blood T cells, FN50 antibody together with TPA synergistically increases cell volume and RNA/DNA synthesis. Addition of FN50 to stimulated peripheral blood B cells has similar, but weaker effect.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Anti-µ-stimulated human B lymphocytes
• Specificity: The antibody recognizes CD69, an lymphocyte early activation marker detected as a 53 kDa band under nonreducing and as 29 + 32 kDa double band under reducing SDS-PAGE conditions.
• Formulation: PBS, pH~7.4
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 969 Human
• Background: CD69 (C-type lectin domain family 2 C, CLEC2C, also known as AIM) is one of the earliest inducible cell surface molecules acquired during leukocyte activation. This glycoprotein serves as a lectin-type receptor in lymphocytes, NK cells and platelets; it is involved in lymphocyte proliferation. CD69 expression is counteracted on T cells in the AIDS stage of HIV infection, and may be also predictive for clinical response to chemoimmunotherapy.
• Synonyms: GP32/28, Leu-23, MLR-3, EA1, BL-AC/P26, AIM