AHNAK Mouse Monoclonal Antibody [Clone ID: EM-09]

CAT#: AM03134PU-N

AHNAK mouse monoclonal antibody, clone EM-09, Purified

Product Images

Immunohistochemistry staining (frozen sections) of murine tongue by anti-AHNAK1 antibody (EM-09; red). Actin filaments were decorated by phalloidin (green), cell nuclei stained with DAPI (blue).

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  Immunofluorescence staining of AHNAK1 in human primary fibroblasts using anti-AHNAK1 (EM-09; green). Actin filaments were decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

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  Immunofluorescence staining of AHNAK1 in HeLa cell line using anti-AHNAK1 (EM-09; red). Cell nuclei stained with DAPI (blue).

Specifications

Product Data

• Clone Name: EM-09
• Applications: IF, IHC, IP, WB
• Recommended Dilution: Western Blot.
  Positive Control: HeLa Human cervix carcinoma cell line.
  Immunocytochemistry (Permeabilization is required).
  Positive Control: HeLa Human cervix carcinoma cell line.
  Immunoprecipitation.
  Immunohistochemistry on Frozen Sections.
  
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Bacterially expressed fragment of N-terminal domain of Human AHNAK1
• Specificity: The antibody reacts with AHNAK1, a 700 kDa multi-functional adaptor protein expressed mainly in epithelial cell, various types of muscle cells and immune cells.
• Formulation: PBS, pH~7.4
  State: Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Protein A Affinity Chromatography
• Database Link:
  Entrez Gene 79026 Human
• Background: AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear; it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.
• Synonyms: Desmoyokin