CD40 Mouse Monoclonal Antibody [Clone ID: HI40a]

CAT#: AM03135FC-N

CD40 mouse monoclonal antibody, clone HI40a, FITC

Product Images

Surface staining of human peripheral blood cells with anti-human CD40 (HI40a) FITC. Cells in the lymphocyte gate were used for analysis.

Specifications

Product Data

• Clone Name: HI40a
• Applications: FC
• Recommended Dilution: Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10e6 cells in a suspension.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human CD40a
• Specificity: This antibody recognizes CD40 (BP50), a 48 kDa type I single chain transmembrane glycoprotein expressed on normal and neoplastic B cells, but not on terminally differentiated plasma cells. CD40 antigen is also present on Hodgkin's and Reed-Sternberg cells, follicular dendritic cells, some macrophages, basal epithelial cells and endothelial cells.
• Formulation: Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent
  Label: FITC
  State: Liquid Ig fraction
  Label: Conjugated with Fluorescein isothiocyanate under optimum conditions. Free of unconjugated
• Conjugation: FITC
• Storage: Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 958 Human
• Background: CD40 is a costimulatory molecule of the TNF receptor superfamily and is expressed on many cell types, such as B cells, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts or vascular smooth muscle cells. Interaction of CD40 and its ligand CD154 (CD40L) is required for the generation of antibody responses to T-dependent antigens as well as for the development of germinal centers and memory B cells. In monocytes/macrophages CD40 engagement induces production of pro-inflammatory cytokines and chemokines. CD40-CD154 interactions are also critical for development of CD4 T cell-dependent effector functions. CD40 links innate and adaptive immune responses to bacterial stimuli and serves as an important regulator affecting functions of other costimulatory molecules.
• Synonyms: TNFRSF5, CDw40, Bp50