ICAM1 Mouse Monoclonal Antibody [Clone ID: 1H4]

CAT#: AM03205RP-N

ICAM1 mouse monoclonal antibody, clone 1H4, PE

Product Images

Surface staining of U937 human histiocytic lymphoma cell line with anti-human CD54 (1H4) PE. Total viable cells were used for analysis.

Specifications

Product Data

• Clone Name: 1H4
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 20 µl to label 10e6 cells or 100 µl whole blood or blood or 10e6 cells in a suspension.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Raji cells and spleen cells fused with NS1 cells
• Specificity: The antibody reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.
• Formulation: PBS
  Label: PE
  State: Liquid purified IgG fraction
  Stabilizer: 0.2% (w/v) high-grade BSA (Protease free)
  Preservative: 15 mM Sodium Azide
  Label: R-Phycoerythrin
• Purification: Size-Exclusion Chromatography
• Conjugation: PE
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: intercellular adhesion molecule 1
• Database Link:
  Entrez Gene 3383 Human
• Background: CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting
  monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
• Synonyms: ICAM-1
• Note: The content of a vial (2 ml) is sufficient for 100 tests.