IL12B Mouse Monoclonal Antibody [Clone ID: CC301]

CAT#: AM03219LE-N

IL12B mouse monoclonal antibody, clone CC301, Low Endotoxin

Specifications

Product Data

• Clone Name: CC301
• Applications: ELISA, Elispot, FC, FN, WB
• Recommended Dilution: ELISA: 8 µg/ml.
  This antibody may be used as a capture antibody in a Sandwich ELISA in combination with Biotinylated clone CC326 (Cat.-No AM05876BT-N) as detection reagent (See Bannerman, D.D.et al.)
  ELISpot.
  Western Blot: 1/50-1/100.
  Flow Cytometry: Use 10 µl of 1/10 diluted antibody to label 10⁶ cells in 100 µl. Membrane permeabilisation is required.
  Functional Assays: 1/100.
• Reactivities: Bovine, Human, Sheep
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant Bovine IL-12.
  Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
• Specificity: This antibody recognises the p40 subunit of Bovine Interleukin-12, and binds to both the free subunit and the intact heterodimer.
  Negative Species: Mouse.
• Formulation: PBS
  State: Low Endotoxin
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Stabilizer: None
  Preservative: None
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G
• Database Link:
  Entrez Gene 281857 Bovine
• Background: Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10; homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35.
• Synonyms: Interleukin-12B, IL-12B, IL-12 subunit p40, CLMF p40, NKSF2