CD2 Mouse Monoclonal Antibody [Clone ID: T6.3]

CAT#: AM05169AC-N

CD2 mouse monoclonal antibody, clone T6.3, APC

Specifications

Product Data

• Clone Name: T6.3
• Applications: FC
• Recommended Dilution: Suitable for Monitoring of T cells in peripheral blood, Analysis of NK subsets, Study of T cell activation.
  Flow cytometry.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with T Lymphocytes activated by mixed lymphocyte culture.
• Specificity: This antibody is analyzed in multi-color with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm.
• Formulation: PBS with 0.08% sodium azide as preservative and 0.2% protein carrier.
  Label: APC
  State: Liquid IgG fraction.
  Label: Allophycocyanin
• Concentration: lot specific
• Conjugation: APC
• Storage: Store the antibody (undiluted) at 2-8°C.
  Do Not Freeze!
• Stability: Shelf life: One year from despatch.
• Gene Name: CD2 molecule
• Database Link:
  Entrez Gene 914 Human
• Background: Identification of human T cells and subset of NK cells associated with the receptor for sheep erythocytes rosettes expressing the 45-50,000 M.W. surface antigen.
• Synonyms: SRBC, Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor
• Note: Protocol: PBMC:
  Add 10 ul of Anitbody/10e6 PBMC in 100 ul PBS.
  Mix gently and incubate for 15 minutes at 2-8°C.
  Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
  WHOLE BLOOD:
  Add 10 ul of Antibody/100 ul of whole blood.
  Mix gently and incubate for 15 minutes at room
  temperature 20°C.
  Lyse the whole blood.
  Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
  See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a Flow cytometer or microscope.