CD3E Mouse Monoclonal Antibody [Clone ID: M2AB]

CAT#: AM05170AC-N

CD3E mouse monoclonal antibody, clone M2AB, APC

Specifications

Product Data

• Clone Name: M2AB
• Applications: FC
• Recommended Dilution: Flow Cytometry.
  This antibody is suitable for Monitoring of T cells subsets in peripheral blood, Characterization of subtypes of T cell leukemia's and lymphomas, Studies of AIDS/HIV virus infection, Analysis of CD3 complex related to the T cell antigen receptor.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: CD3=Derived from the hybridization of mouse NS-1 myeloma celss with spleen cells from BALB/c mice immunized with human thymocytes.
• Formulation: PBS with 0.08% sodium azide as preservative and 0.2% protein carrier.
  Label: APC
  State: Liquid (sterile filtered) IgG fraction.
  Label: analyzed in multi-color flow cytometry with instrumentsequipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe)laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm
• Concentration: lot specific
• Conjugation: APC
• Storage: Store the antibody (undiluted) at 2-8°C.
  Do Not Freeze!
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 916 Human
• Background: The CD3 epitope is expressed on the epsilon chain of the CD3/T cell antigen receptor (TcR) complex.
  CD3 is present on 65-85% of thymocytes and has a mitogenic effect on peripheral blood T cells.
  Identification of human T cells expressing the 22-28,000 M.W. surface antigen.
• Synonyms: T3/Leu-4
• Note: Protocol: PBMC:
  Add 10 ul of Antibody/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C.
  Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
  
  WHOLE BLOOD:
  Add 10 ul of Antibody/100 ul of whole blood.
  Mix gently and incubate for 15 minutes at room temperature 20°C.
  Lyse the whole blood.
  Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
  See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.