CD5 Mouse Monoclonal Antibody [Clone ID: M28323]

CAT#: AM05171AC-N

CD5 mouse monoclonal antibody, clone M28323, APC

Specifications

Product Data

• Clone Name: M28323
• Applications: FC
• Recommended Dilution: Flow Cytometry.
  This antibody is suitable for Monitoring of T cells subsets in peripheral blood, Analysis of B cell subsets, Characterization of subtypes of T cell leukemias and lymphomas.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: CD5=Derived from the hybridization of mouse NS-1/Ag4 myeloma cells with spleen cells of BALB/c mice immunized with human t-acute lymphoblastic leukemia (ALL) cells.
• Formulation: PBS containing 0.2% protein carrier and 0.08% Sodium Azide as preservative.
  Label: APC
  State: Liquid purified IgG fraction.
• Concentration: Lot specific
• Conjugation: APC
• Gene Name: CD5 molecule
• Database Link:
  Entrez Gene 921 Human
• Background: Identification of human helper/inducer T cells expressing the 67,000 M.W. surface antigen, 85% peripheral blood lyymphocytes that form rosettes with sheep red blood cells (E+), and a small subset of B cells.
• Synonyms: CD5, LEU1
• Note: Protocol: PBMC:
  Add 10 ul of Antibody/10e6 PBMC in 100 ul PBS.
  Mix gently and incubate for 15 minutes at 2-8°C.
  Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
  
  WHOLE BLOOD:
  Add 10 ul of Antibody/100 ul of whole blood.
  Mix gently and incubate for 15 minutes at room temperature 20°C.
  Lyse the whole blood.
  Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
  See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.