CD8A Mouse Monoclonal Antibody [Clone ID: 17D8]

CAT#: AM05173AC-N

CD8A mouse monoclonal antibody, clone 17D8, APC

Specifications

Product Data

• Clone Name: 17D8
• Applications: FC
• Recommended Dilution: Flow Cytometry.
  Monitoring of T cells subsets in peripheral blood; Analysis of NK cell subsets; Study of cell mediated cytotoxicity.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: CD8=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
• Specificity: This antibody detects human T and NK Lymphocytes.
• Formulation: PBS containing 0.2% protein carrier and 0.08% Sodium Azide as preservative.
  Label: APC
  State: Liquid (sterile filtered) purified IgG fraction.
  Label: Allophycocyanin conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm
• Concentration: lot specific
• Conjugation: APC
• Storage: Store the antibody undiluted at 2-8°C.
  Do Not Freeze!
• Stability: Shelf life: One year from despatch.
• Gene Name: Homo sapiens CD8a molecule (CD8A), transcript variant 1
• Database Link:
  Entrez Gene 925 Human
• Background: Identification of human T cells suppressor/cytotoxic expressing the 32 kDa M.W. surface antigen.
• Synonyms: CD8 alpha chain, CD8A, MAL
• Note: Protocol: PBMC:
  Add 10 ul of MAB/10e6 PBMC in 100 ul PBS.
  Mix gently and incubate for 15 minutes at 2-8°C.
  Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
  
  WHOLE BLOOD:
  Add 10 ul of MAB/100 ul of whole blood.
  Mix gently and incubate for 15 minutes at room temperature 20°C.
  Lyse the whole blood.
  Wash once with PBS and analyze or fix with 0.5% v/v of
  paraformaldehyde in PBS and analyze.
  See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
• Protein Pathways: Antigen processing and presentation, Cell adhesion molecules (CAMs), Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway