CD28 Mouse Monoclonal Antibody [Clone ID: B-23]

CAT#: AM05187AC-N

CD28 mouse monoclonal antibody, clone B-23, APC

Specifications

Product Data

• Clone Name: B-23
• Applications: FC
• Recommended Dilution: Flow Cytometry.
  Monitoring of activated T cells in peripheral blood.
  Study of T lymphocyte cytokine function.
  Study of B cell activation.
  Study of Cell-adhesion molecules relating T and B lymphocytes.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: CD28=Derived from the hybridization of mouse Sp2/O-Ag14 myeloma cells with spleen cells of BALB/c mice immunized with the HPB-ALL T-cell line.
• Specificity: This antibody recats with CD28 receptor.
• Formulation: PBS containing 0.2% protein carrier and 0.08% Sodium Azide as preservative.
  Label: APC
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Conjugation: APC
• Storage: Store the antibody undiluted at 2-8°C.
  Do Not Freeze!
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 940 Human
• Background: Anti-human CD28 binds the 44kDa MW cell surface protein on the surface of most T cells. CD28 acts as the ligand for the B7/BB-1 molecule on the surface of activated B cells. B7/BB-1 costimulates T cells through CD28, along with CD2 and CD3.CD28 antigen is a disulfide-linked homodimeric glycoprotein. The CD28 antigen is present on approximately 60%-80% T lymphocytes (95% of CD4 and 50% of CD8 lymphocytes). CD28 regulates the expression of cytokines by T cells, not only IL-2, but also IL-1 alpha and CSF-1, usually synthesized by accessory cells. CD28 functions as a cell adhesion molecule (CAM) for certain T cell subsets.
• Synonyms: TP44
• Note: Protocol: PBMC:
  Add 10 ul of MAB/10e6 PBMC in 100 ul PBS.
  Mix gently and incubate for 15 minutes at 2-8°C.
  Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
  
  WHOLE BLOOD:
  Add 10 ul of MAB/100 ul of whole blood.
  Mix gently and incubate for 15 minutes at room temperature (20°C).
  Lyse the whole blood.
  Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
  See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.