CD33 Mouse Monoclonal Antibody [Clone ID: FOS]

CAT#: AM05188RP-N

CD33 mouse monoclonal antibody, clone FOS, PE

Specifications

Product Data

• Clone Name: FOS
• Applications: FC
• Recommended Dilution: Flow Cytometry.
  Characterization of subtypes of leukemias and lymphomas.
  Studies of AIDS virus infection.
  Myeloid cell function studies.
  Analysis of hematopoietic maturation.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: CD33=Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with FMY9S5 cells containing the CD33 gene.
• Specificity: This antibody recognises human Myeloid.
• Formulation: PBS containing 0.2% protein carrier and 0.08% Sodium Azide as preservative.
  Label: PE
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Conjugation: PE
• Storage: Store the antibody undiluted at 2-8°C.
  Do Not Freeze!
• Stability: Shelf life: One year from despatch.
• Gene Name: CD33 molecule
• Database Link:
  Entrez Gene 945 Human
• Background: CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.
  Identification of human Monocytes (bright) and Granulocytes (dim) expressing the 67kDa M.W. surface antigen.
  CD33 is also found on CFU-mix, CFU-GM, CFU-Meg, a portion of BFU-E, myeloblasts, promyelocytes, myelocytes, metamyelocytes but not early precursors.
• Synonyms: Siglec-3, gp67
• Note: Protocol: PBMC:
  Add 10 ul of MAB/10e6 PBMC in 100 ul PBS.
  Mix gently and incubate for 15 minutes at 2-8°C.
  Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
  
  WHOLE BLOOD:
  Add 10 ul of MAB/100 ul of whole blood.
  Mix gently and incubate for 15 minutes at room temperature (20°C).
  Lyse the whole blood.
  Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
  See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.