CD45 (PTPRC) Mouse Monoclonal Antibody [Clone ID: BHPT-1]

CAT#: AM05192AC-N

CD45 (PTPRC) mouse monoclonal antibody, clone BHPT-1, APC

Specifications

Product Data

• Clone Name: BHPT-1
• Applications: FC
• Recommended Dilution: Flow Cytometry.
  Monitoring of Leukocyte cell subsets in peripheral blood.
  Characterization of subtypes of leukemia's and lymphomas.
  Study of AIDS virus infection.
  Studies of progenitor cells in bone marrow.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells of BALB/c mice immunized with peripheral blood monocytes from rheumatoid arthritis patient
• Specificity: This antibody reacts with Leukocyte (HLe-1).
• Formulation: PBS containing 0.2% protein carrier and 0.08% Sodium Azide as preservative.
  Label: APC
  State: Liquid purified IgG fraction.
  Label: Allophycocyanin conjugates are analyzed in multi-color Flow Cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: APC
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 5788 Human
• Background: Identification of all human leukocytes, lymphocytes, monocytes, polymorphonuclear cells, eosinophils and basophils in peripheral blood expressing the 180-220 kD M.W. T200 family surface antigen. CD45 is also present on thymus, spleen, tonsil and progenitor cells in bone marrow.
• Synonyms: PTPRC, Leukocyte common antigen, L-CA, T200
• Note: Protocol: PBMC:
  Add 10 ul of MAB/10e6 PBMC in 100 ul PBS.
  Mix gently and incubate for 15 minutes at 2-8°C.
  Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
  
  WHOLE BLOOD:
  Add 10 ul of MAB/100 ul of whole blood.
  Mix gently and incubate for 15 minutes at room temperature (20°C).
  Lyse the whole blood.
  Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
  See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.