HLA Class II DR Mouse Monoclonal Antibody [Clone ID: 423L]

CAT#: AM05204FC-N

HLA Class II DR mouse monoclonal antibody, clone 423L, FITC

Specifications

Product Data

• Clone Name: 423L
• Applications: FC
• Recommended Dilution: Flow Cytometry.
  Monitoring of B cells in peripheral blood.
  Analysis of B subsets.
  Study of B cell activation.
  Study of B cell neoplasms.
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Derived from the hybridization of mouse NS-1/1-Ag4 myeloma cells with spleen cells of BALB/c mice immunized with human lymphoblastoid B-cell line RPMI 8866.
• Specificity: This antibody recats with Human B and T subset Lymphocyte. Other species not tested.
• Formulation: PBS containing 0.2% protein carrier and 0.08% Sodium Azide as preservative.
  Label: FITC
  State: Liquid purified IgG fraction
• Concentration: Lot specific
• Purification: Protein A/G Chromatography
• Conjugation: FITC
• Background: Identification of human B cell and T cell subsets associated with approximately 10% of peripheral blood lymphocytes 28-34,000 M.W. surface antigen, also low density on monocytes and macrophages.
• Synonyms: HLA-DR, HLA class II histocompatibility antigen DR, MHC class II antigen DR
• Note: Protocol: PBMC:
  Add 10 ul of MAB/10e6 PBMC in 100 ul PBS.
  Mix gently and incubate for 15 minutes at 2-8°C.
  Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
  
  WHOLE BLOOD:
  Add 10 ul of MAB/100 ul of whole blood.
  Mix gently and incubate for 15 minutes at room temperature (20°C).
  Lyse the whole blood.
  Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
  See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.