Integrin alpha E (ITGAE) Mouse Monoclonal Antibody [Clone ID: AX.14]

CAT#: AM05205FC-N

Integrin alpha E (ITGAE) mouse monoclonal antibody, clone AX.14, FITC


USD 270.00

5 Days*

Size
    • 100 Tests

Product Images

Specifications

Product Data
Clone Name AX.14
Applications FC
Recommended Dilution Flow Cytometry.
Monitoring of Mucosa-associated T Lymphocytes.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Specificity This antibody reacts with Intestinal Lymphocytes (HML-1).
Formulation PBS containing 0.2% protein carrier and 0.08% Sodium Azide as preservative.
Label: FITC
State: Liquid purified IgG fraction.
Label: Fluorescein Isothiocyanate Isomer 1
Concentration lot specific
Conjugation FITC
Storage Store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
This product is photosensitive and should be protected from light.
Stability Shelf life: One year from despatch.
Background The CD103 antigen is a 160-130-105 kDa molecule which is expressed on mucosa-associated T lymphocytes and activated cells and on subset of TGF beta -1 cells. This human intra epithelial lymphocyte marker is the E integrin chain associated with the b7 subunit. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood ymphocytes. CD103 is expressed by 0.5 to 2% of resting lymphocytes in Peripheral blood and lymphoid organs.
Synonyms Integrin alpha-E, Integrin alpha-IEL, HML-1 antigen, ITGAE
Note Protocol: PBMC:
Add 10 µl of MAB/10e6 PBMC in 100 µl PBS.
Mix gently and incubate for 15 minutes at 2-8°C.
Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.

WHOLE BLOOD:
Add 10 µl of MAB/100 µl of whole blood.
Mix gently and incubate for 15 minutes at room temperature (20°C).
Lyse the whole blood.
Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.