CD11a (ITGAL) Mouse Monoclonal Antibody [Clone ID: OFYS]

CAT#: AM05207BT-N

CD11a (ITGAL) mouse monoclonal antibody, clone OFYS, Biotin


USD 270.00

5 Days*

Size
    • 100 Tests

Product Images

Specifications

Product Data
Clone Name OFYS
Applications FC
Recommended Dilution Flow Cytometry.
Characterization of LFA-1 immunodeficiency.
Monitoring of subsets in peripheral blood.
Graft-versus-host disease.
Analysis of CTL-mediated cytotoxicity.
Studies of transplantation rejection.
Functional studies of cell adhesion molecules.
Reactivities Human
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Specificity This antibody reacts with LFA-1 alpha.
This antibody blocks cell-cell adhesion and adhesion mediated events such as cell mediated cytotoxicity.
Formulation PBS containing 0.2% protein carrier and 0.08% Sodium Azide as preservative
Label: Biotin
State: Liquid purified IgG fraction.
Concentration lot specific
Conjugation Biotin
Storage Store the antibody undiluted at 2-8°C.
Do Not Freeze!
Stability Shelf life: One year from despatch.
Gene Name integrin subunit alpha L
Background CD11a, Leukocyte Function Antigen-1 (LFA-1) complex is a heterodimeric glycoprotein consisting of two non-covalently linked chains, a 180 kDa alpha chain and a 95 kDa beta chain. It is a member of the integrin supergene family of adhesion molecules, and is on virtually all peripheral blood leukocytes. The molecule binds to ICAM-1, 2 and 3 and mediates cell-cell adhesion.
Synonyms Integrin alpha-L, LFA1, LFA-1
Note Protocol: PBMC:
Add 10 ul of MAB/10e6 PBMC in 100 ul PBS.
Mix gently and incubate for 15 minutes at 2-8°C.
Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.

WHOLE BLOOD:
Add 10 ul of MAB/100 ul of whole blood.
Mix gently and incubate for 15 minutes at room temperature (20°C).
Lyse the whole blood.
Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.